Home Science & Technology Optimize qPCR through standardization

Optimize qPCR through standardization


Real-time quantitative PCR (qPCR) is very popular, but it has resulted in a wide range of protocols, reagents, cycle steps, analysis techniques, and reporting formats. The lack of uniformity and consensus hinders the accuracy, consistency, and cross-application of qPCR data, which is further complicated by many scientific papers that obscure key information. Thus, standardization, especially since qPCR workflows include automation, is vital to the consistency, reproducibility, and dissemination of qPCR data.

Download this ebook from Bio-Rad and Scientist The Creative Services team to learn about

  • Why qPCR standardization is necessary for experimental reproducibility
  • Creating a universal qPCR standard for storing and disseminating data
  • Incorporate automation into qPCR workflows without compromising standardization
  • An example of an automated qPCR workflow
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